Bactria and disinfectants savlon

                            Bactria  and disinfectants 

purpose : how concentration can affect the performance rate of disinfectants in killing micro-organism

hypothesis: I predict that the higher the concentration of the disinfectant ,the higher the rate of killing off the bacteria.

equipment:

Agar plate 

marker pen 

filter paper 

hole puncher 

10ml measuring cylinder

Dettol

ruler 

bacteria 

cotton bud 

plastic pipette 

tweezers
Ethanol 

method:

1. With a permanent marker ,label your agar plate as a quadrant (see diagram) 

2. Take a cotton bud ,dip it into the yoghurt and the yoghurt is in a breaker.

Spread evenly across the agar plate. 

3. Use a plastic spotting tile and a pipette  and Take the Savlon and diluted the Savlon 

- for %0 Savlon  put water in the first hole in the spotting tile and use the pipette to drop the water  ,10 drops. This is called  "control"

-for 1:10 savlon  , take  one drop of savlon that you used in the first spotting tile hole  and put that one drop in a second hole ,put 9 drops of water in the second tile.

-for 1:100 savlon  , take one drop of the 1:10 solution then put the one drop into the third tile and then put 9 more drops of water in the third tile.
-for 1:1000 savlon , take one drop of 1:100 solution next put the one drop into the fourth tile and then put 9 drops of water in the fourth tile.

4. Get the filter paper to hole punch 4 circles .

5. Turn on the Bunsen burner. Get the tweezers and put the top of the tweezers in Ethanol then run it over the flame to sterilizes the tweezers.

6. Pick up the circles with the tweezers and put the first circle in the  %100/control  savlon  ,put it in the first quadrant then sterilizes the tweezers again and put the second circle in the 1:10  , put it in the second quadrant then do the same step with the 1:100 and the 1:1000.

7. Put the lid on the agar plate and leave it for 24 hours.

research:    

*bacteria 

*choroxylenol 

*what does choroxylenol do to bacteria 

*bacteria life cycle 

* dettol -how does it work -what is it -what is disinfectant. 

Chloroxylenol, also known as para-chloro-meta-xylenol , is an antiseptic and disinfectant which is used for skin disinfection and cleaning surgical tools . It is also used within a number of household disinfectants eg floor , window , bath etc and wounds cleaner. Chloroxylenol is most effective against Gram-positive bacteria. https://en.wikipedia.org/wiki/Chloroxylenol.

Bactria a member of a large group of unicellular microorganisms which have cell walls but lack organelles and an organized nucleus, including some which can cause disease.

gram-negative bacteria have a very thin peptidoglycan layer that is sandwiched between an inner cell membrane and a bacterial outer membrane.http://fermup.com/blog/what-does-gram-positive-mean/

Bacteria life cycle